<div id="bm-composer-content-wrapper"><div id="bm-composer-content-wrapper"><div id="bm-composer-content-wrapper"><div style="font-family: "Montserrat", sans-serif; font-size: 9pt; color: rgb(31, 31, 31);"><pre>We would like to draw your attention to an opening for a PhD position in
Grenoble, France, between the Institut de Biologie Structrale (IBS) and
the Institut Laue Langevin (ILL).
The selected candidate will perform modelling by Alphafold, cryo-EM on a challenging biological
target (see detailed project below) in the MICA group at IBS, as well as
undergo neutron scattering experiments at the ILL.
The candidates are expected to have a solid background in biophysics, biochemistry and structural
biology. Previous experience in cryo-EM and/or neutron scattering would
be a plus, but not required.
Please send a CV, a motivation letter and the contact details of at
least two referees to the two co-supervisors of this position :
Dr. Ambroise Desfosses (IBS) : <a>ambroise.desfosses@ibs.fr</a>
Prof. Judith Peters (ILL) : <a>jpeters@ill.fr</a>
The position has to start before end of March, applications will be
accepted until mid-February.
Project description :
The natural nanoparticle "low density lipoprotein" (LDL) is responsible
for the transport of cholesterol in the blood. Dysfunction of this
functionality can lead to serious diseases. It contains a unique
protein, apoB-100, which has a molar mass of 500 kDa. While the whole
particle and its protein have been studied for a long time, it has still
not been possible to determine their structures at the atomic level.
To obtain new models of LDL and apo B-100 with an unprecedented level of
detail we will pursue three individual paths that will converge: LDL will
be investigated, and independently its sole protein moiety apo B-100 in
a lipid-free detergent-stabilized form. To access their structures we
will combine modelling by Alphafold and simulations,<br>state-of-the-art cryo-EM techniques, SAXS, and neutron
scattering methods. All methods will be applied not only on LDL and/or
its subfractions, but also on triglyceride-rich and oxidized LDL to
mimic pathologic conditions as found in hyperlipidaemia or in
atherosclerotic plaques.</pre><br></div><div data-bm-signature="default"><pre></pre></div></div></div><style></style></div>